Research focus

Optimization and development of MALDI imaging mass spectrometry (MALDI IMS) protocols to satisfy specific research needs is our main focus and opportunity for collaborative projects.  

We also focus on development and optimization of methods using gas chromatography-mass spectrometry (GC-MS) for characterization of volatile metabolites/markers produced by cell cultures, tissues and other biological samples.

Research objectives

  • Molecular imaging of neurotransmitters in brain tissue sections
  • MALDI-IMS mapping of tumor-associated proteins
  • MALDI IMS mapping of pharmaceuticals and their metabolies in biological tissues
  • Application of GC-MS for characterization of volatile markers of inflammatory disorders

Main partners

  • TGen, the Translational Genomics Research Institute, Patrick Pirrotte PhD, Assistant Professor and Director of Collaborative Center for Translational Mass Spectrometry.
  • Tel Aviv University, Department of Neurobiology, Dan Frenkel PhD, Laboratory Head, Assistant Professor.
  • University of Applied Sciences Technikum Wien, Andreas Teuschl PhD, Head of Competence Team SignalTissue.
  • Institute of Organic Chemistry and Biochemistry of the CAS, Mass Spectrometry Research-Service Group, Martin Hubálek PhD, Research Scientist.
  • Technische Universität Berlin, Department Food Chemistry and Toxicology, Dr. Claudia Keil.

Offered services and expertise

  • Development of MALDI IMS compatible protocols for formalin-fixed paraffin embedded tissues.
  • Protein identification in complex mixtures.
  • Relative quantification in complex mixtures.
  • (Co)-IP-MS.
  • MALDI‐TOF profiling.
  • MS data analysis and interpretation.
  • GC-MS analyses of volatile metabolites.

Top publications

  • Co-authorship in manuscript CIRCRES/2019/315980 submitted for publication in Circulation Research.
  • Vanickova L, Guran R, Kollar S, Emri G, Krizkova S, Do T, Zitka O, Heger Z, Adam V. 2019. Mass spectrometric imaging of cysteine rich proteins in human skin. International Journal of Biological Macromolecules. 125, 270-277. https://doi.org/10.1016/j.ijbiomac.2018.11.272.
  • Guran R, Vanickova LP, Horak V, Krizková S, Michalek P, Heger Z, Zitka O, Adam V. 2017. MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles. PLOS ONE. 12:1-15. ISSN: 1932-6203.

Other selected results

  • Vanickova L, Pavelicova K, Zemankova K, Pompeiano A, Mravcova L, Bendickova K, Kohoutkova HM, Fric J. Mass Spectrometry: Applications to the Clinical Lab 2019.
  • Vaneckova T, Vanickova LP, Pomorski A, Vaculovic T, Krezel A, Vaculovicova M, Adam V. 2019. Talanta. 198, 224-229. https://doi.org/10.1016/j.talanta.2019.01.089.
  • Tvrdonova M, Vlcnovska M, Vanickova LP, Kanicky V, Adam V., Ascher L., Jakubowski N, Vaculovicova M, Vaculovic T. 2019. Analytical and Bioanalytical Chemistry. 411 (3), 559-564.
  • Strmiska V, Michalek P, Lackova Z, Guran R, Krizkova S, Vanickova L, Zitka O, Stiborova M, Eckschlager T, Klajdus B, Pacik D, Tvrdikova E, Haase H, Adam V, Heger Z. Molecular Oncology. doi: 10.1002/1878-0261.12439.

Team members

  • Martin Eugenio Barrios-Llerena, Ph.D.
  • Mgr. Ondřej Polanský
  • Romana Vlčková
  • Mgr. Sabina Gomelská
  • Ing. Lucie Pompeiano Vaníčková, Ph.D.
  • Ing. Monika Siekelová
  • Mgr. Zuzana Trávníčková
  • Mgr. Tereza Virglová

Principal Investigator

Martin Eugenio Barrios-Llerena, Ph.D.
Martin Eugenio Barrios-Llerena, Ph.D.
Head of CF
Phone: +420 54 318 511158281