Research focus

Inflammation is a fundamental response of virtually all multicellular organisms to infection and injury. This research group is focused on elucidation of molecular mechanisms underlying acute and chronic inflammatory processes, particularly on regulatory role of phagocytes and development of vascular inflammation. In collaboration with chemists and pharmacologists the group investigates potential of different compounds such as nitro-lipids and hyaluronan derivatives as new anti-inflammatory drugs. Furthermore, the Inflammation group studies biological importance of different isoforms of adenylate cyclases and a potential of new compounds specifically modulating their activities as prospective anti-inflammatory drugs.

Research objectives

  • Identification of molecular mechanisms responsible for development of endothelial dysfunction and tissue damage under conditions of acute and chronic inflammation including ischemic insults.
  • Development of new anti-inflammatory drugs targeting cAMP signaling pathways particularly specific isoforms of adenyl cylases.
  • Identification of regulatory role of phagocytes and their newly defined subpopulations in the course of acute and chronic inflammation.

Main partners

  • University of Cologne, Cologne, Germany
  • University of California, Davis, CA, USA
  • University of Pittsburgh, Pittsburgh, PA, USA

Technological equipment

  • Microfludic systems mimicking vasculature in vitro.
  • Intravital microscopy system equipped with spinning disk confocal unit for observation of leukocyte-endothelium interactions in vivo.
  • High throughput cell free and cell based screens for determination of modulation of specific isoform of adenylate cyclase activity.
  • High throughput cell based assay for determination of potential to activate specific toll like receptor.
  • Set of instruments for determination of production of various reactive oxygen species, nitric oxide, and antioxidative capacity in various biological samples.

Offered services and expertise

  • Testing anti-inflammatory drugs in in vitro models and evaluation of their efficiency in variety of preclinical animal models.
  • Studies employing endothelial cell seeded microfluidic systems that mimic microvascular system.
  • Testing biocompatibility and regenerative potential of new materials and compounds in vitro and in vivo.

Top publications

  • KOLAROVA, H., KLINKE, A., KREMSEROVA, S., ADAM, M., PEKAROVA, M., BALDUS, S., EISERICH, J., KUBALA, L. Myeloperoxidase induces the priming of platelets. Free Radical Biology and Medicine. 2013, 61(April), 357-369.
  • ADAM, M., GAJDOVÁ, S., KOLÁŘOVÁ, H., KUBALA, L., LAU, D., GEISLER, A., RAVEKES, T., RUDOLPH, V., TSAO, P., BLANKENBER, S., BALDUS, S., KLINKE, A. Red blood cells serve as intravascular carriers of myeloperoxidase. Journal of Molecular and Cellular Cardiology. 2014, 74(September), 353-63.
  • AMBROZOVA, G., MARTISKOVA, H., KOUDELKA, A., RAVEKES, T., RUDOLPH, T., KLINKE, A., RUDOLPH, V., FREEMAN, B., WOODCOCK, S., KUBALA, L., PEKAROVÁ, M. Nitro-oleic acid modulates classical and regulatory activation of macrophages and their involvement in pro-fibrotic responses. Free Radical Biology and Medicine. 2016, 90(January), 252-260.
  • Ambrozova, G., Fidlerova, T., Verescakova, H., Koudelka, A., Rudolph, T.K., Woodcock, S.R., Freeman, B.A., Kubala, L., Pekarova, M. Nitro-oleic acid inhibits vascular endothelial inflammatory responses and the endothelial-mesenchymal transition. Biochimica et Biophysica Acta. 2016. [Epub ahead of print]
  • Kudová, J., Procházková, J., Vašiček, O., Perečko, T., Sedláčková, M., Pešl, M., Pacherník, J., Kubala, L.. HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. PLoS One. 2016, 29, 11(6), e0158358.

Other selected results

  • Significance of phagocyte derived enzyme myeloperoxidase in the pathogenesis of vascular dysfunction and cardiovascular diseases.
  • Mechanisms of function and therapeutic use of nitro-fatty acids as cardioprotective and anti-inflammatory mediators.
  • Determination of effect of hypoxia on differentiation of embryonic stem cells and role of HIF signalling in cardiac differentiation and regeneration.

Team members

  • Mgr. Lukáš Kubala, Ph.D.
  • Mgr. Gabriela Ambrožová, Ph.D.
  • Mgr. Kristýna Turková, Ph.D.
  • Mgr. Jan Víteček, Ph.D.
  • Mgr. Marek Černík
  • Leticia Dobler, MSc
  • Mgr. Jitka Glossová
  • Bc. Anna Kocurková
  • Bc. Michaela Pešková
  • Ing. Iva Zaplatilová

Principal Investigator

Mgr. Lukáš Kubala, Ph.D.
Mgr. Lukáš Kubala, Ph.D.
Principal Investigator

Assistant

Mgr. Kristýna Turková, Ph.D.
Mgr. Kristýna Turková, Ph.D.
Postdoctoral Researcher