The overall goal of research of Cell and Tissue Regeneration group is to investigate the properties of human stem cells that are relevant to their utility in biomedicine. Particular focus is on pluripotent stem cells, both embryonic and induced, but adult stem cell types are also studied. The biological phenomena that are being studied mainly include: a) genomic stability of stem cells because of its importance to safety in potential clinical applications, b) role of cell cycle regulators and non-coding RNAs in establishing and maintenance of differentiated phenotypes and also in pluripotency, and c) effects of extracellular biodomains on behavior of stem cells, with an emphasis on their contribution to establishment of niche environment. Great effort is also dedicated to understanding of stem cell-to-extracellular matrix interactions and to development of methodologies for supporting growth of cells in engineered scaffolds as well as differentiation of stem cells towards defined somatic cell lineages (e.g. airway epithelia).
Live cell imaging microscopy; transmission and scanning electron microscopy; stem cell phenotyping; establishment, propagation, and differentiation of human pluripotent stem cells; mass spectrometry-based cell fingerprinting.
BARTA, T., PESKOVA, L., HAMPL, A. miRNAsong: a web-based tool for generation and testing of miRNA sponge constructs in silico. Sci Rep. 2016, 6:36625.
STREIT, L., JAROS, J., SEDLAKOVA, V., SEDLACKOVA, M., DRAZAN, L., SVOBODA, M., POSPISIL, J., VYSKA, T., VESELY, J., HAMPL, A. A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting. Plast Reconstr Surg. 2017, 139:670e-682e.
VANHARA, P., KUCERA, L., PROKES, L., JURECKOVA, L., PENA-MENDEZ, E.M., HAVEL. J., HAMPL, A. Intact Cell Mass Spectrometry as a Quality Control Tool for Revealing Minute Phenotypic Changes of Cultured Human Embryonic Stem Cells. Stem Cells Transl Med. 2018 7:109-114.
POROKH, V., VANHARA, P., BARTA, T., JURECKOVA, L., BOHACIAKOVA, D., POSPISILOVA, V., MINARIKOVA, D., HOLUBCOVA, Z., PELKOVA, V., SOUCEK, K., HAMPL, A. Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells. Stem Cells Dev. 2018, 27:1077-1084.